GLP-1 ELISA (Glucagon-Like Peptide-1)

This kit is for determination of biologically active forms of Glucagon-Like Peptide-1 [i.e. GLP-1 (7-36 amide) and GLP-1 (7-37)] in plasma and other biological media. It is highly specific for the immunologic measurement of active GLP-1 and will not detect other forms of GLP-1 (e.g., 1-36 amide, 1-37, 9-36 amide, or 9-37). The GLP-1 sequence is highly conserved between the species, with no sequence variation occurring at all in mammals.

Regulatery Status: RUO
Catalog No Size
Product Catalog No: EIA-3056 Pack Size: 96 Wells

Pack Size:
Pack Size:
Pack Size:
Pack Size:

Category:
Test Principle

This assay is based, sequentially, on: 1) capture of active GLP-1 from sample by a monoclonal antibody, immobilized in the wells of a microwell plate, that binds specifically to the N-terminal region of active GLP-1 molecule, 2) washing to remove unbound materials, 3) binding of an anti GLP-1-alkaline phosphatase detection conjugate to the immobilized GLP-1, 4) washing off unbound conjugate, and 5 ) quantification of bound detection conjugate by adding MUP (methyl umbelliferyl phosphate) which in the presence of alkaline phosphatase forms the fluorescent product umbelliferone. Since the amount of fluorescence generated is directly proportional to the concentration of active GLP-1 in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of active GLP-1.

Order Enquiry

Order Enquiry Form

References
  • Nathan DM, Schreiber E, Fogel H, Mojsov S, Hebener JF. Insulinotropic Action of Glucagon-like peptide-1 (7-37) in Diabetic and Nondiabetic Subjects. Diabetes Care 15: 270-276, 1992
  • Kieffer TJ, McIntosh CHS, Pederson RA. Degradation of Glucose-Dependent Insulinotropic Polypeptide (GIP) and Truncated Glucagon-Like Peptide (GLP-1) in vitro and in vivo by Dipeptidyl Peptidase IV. Endocrinology 136: 3585-3596, 1995
  • Tijsen P. “Practice and Theory of Enzyme Immunoassays” in Burdon RH and Knippenberg PH (Ed.), Laboratory Techniques in Biochemisrty and Molecular Biology. Amsterdam/NY: Elsevier, 1985
  • Christopoulos TK and Diamandis EP. “Fluorescence Immunoassays” in Diamandis EP and Christopoulos TK (Ed.), Immunoassay. Academic Press, 1996
  • Holst JJ, Cathrin Orskov, Bolette Hartmann, Carolyn F. Deacon : Postranslational processing of proglucagon and postsecretory fate of proglucagon products; in Fehmann HC, Goke B (eds) : The Insulinotropic Gut Hormone Glucagon-Like-Peptide-1. Front Diabetes. Basel, Karger, 1997, vol 13, pp 24-48
Documents
Enquiry