Carbon Dioxide (CO2) Assay

Carbon Dioxide Enzymatic Assay Kit is intended for the quantitative determination of carbon dioxide (CO2) in serum and plasma

Regulatery Status: FDA 510(k), CE

Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (300 Tests) Pack Size: DZ122A-K Pack Size: R1: 5 x 20 mL
Cal: 1 x 2 mL vial
(Calibrator Included)
Pack Size: Colorimetric/Kinetic
Product Catalog No: Kit (1200 Tests) Pack Size: DZ122A-KB1 Pack Size: R1: 4 x 100 mL
Product Catalog No: Kit (1680 Tests) Pack Size: DZ122A-KY2 Pack Size: R1: 10 x 56 mL
Product Catalog No: Calibrator Pack Size: DZ122A-CAL Pack Size: Cal:1 x 2 mL vial
Pack Size:
Product Catalog No: Control Pack Size: DZ122A-CON Pack Size: Con: 2 x 2 mL vial
Pack Size:

Category:
Product Features

CO2 Enzymatic Assay is a single vial liquid stable reagent system which has a neutral pH, minimizing the effect of CO2 absorption from the air. In addition to a highly stable NADH analog as a co-factor the reagent maintains integrity by rebuilding key reagent components as required for optimal performance. The assay has a linearity of 1.12 – 50 mmol/L, correlation coefficient value of 0.99, slope of 1.0447, y intercept of -0.9742 and is highly precise with Intra and Inter Assay Precision %CV’s of <3.5%. Packaged for optimal operator convenience, reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including RocheTM Hitachi 917 series, Beckman AU (400/600/640/680), Beckman Synchron CX, LX and DXC.

Assay Principle

Carbon Dioxide Enzymatic Assay is based on two coupled enzyme reactions including phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH). PEPC catalyzes the first reaction which produces oxaloacetate. In the presence of MDH, the reduced cofactor is oxidized by oxaloacetate. This results in a decrease of absorbance at 405 or 415 nm that is directly proportional to CO2 concentration in the sample.

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