Anti-Phospholipid Screen IgG/IgM
The Anti-Phospholipid Screen IgG/IgM assay is an enzyme immunoassay (EIA) intended to detect the presence of IgG and IgM class autoantibodies against cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and β2-Glycoprotein I in human serum or plasma.
A mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human β2-glycoprotein I is bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG or IgM immunologically detects the bound antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm.
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