Alkaline phosphatase Assay Kit (ALP)

For the in vitro quantitative determination of alkaline phosphatase (ALP) in serum.



Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (500 Ml) Pack Size: GB040G Pack Size: R1: 4 ×100 ml
R2: 2 × 50 ml
Pack Size: IFCC
Product Catalog No: Kit (450 Ml) Pack Size: GS041G Pack Size: R1: 6 × 60 ml
R2: 2 × 45 ml
Product Catalog No: Kit (75 Ml) Pack Size: GH041G Pack Size: R1: 2 × 50 ml
R2: 1 × 25 ml
Product Catalog No: Kit (300 Ml) Pack Size: GT041G Pack Size: R1: 5 × 48 ml
R2: 2 × 30 ml
Product Catalog No: Kit (200 Ml) Pack Size: GX041G Pack Size: R1: 2 × 80 ml
R2: 2 × 20 ml
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Product Features

Alkaline phosphatase is a membrane-bound enzyme which is present in most tissues. It has three different isoenzymes derived from small intestine-placentabone/liver/kidney. It is a dimer molecule containing Zn2+ ions, which play a role in the maintenance of structure and catalysis. The enzyme found in human serum is derived from bone, liver and small intestine. During pregnancy the enzyme from the placenta dominates (it is heat stable at 65°C). In the past the isoenzymes were separated using various inhibitors and heat. The role of electrophoresis is growing in determining the concentrations. The increase in enzyme activity is prevalent in various hepatic and bone decrease states. The level is also increased in certain diseases of the thyroid gland, intestinal tract and in several bacterial infection.

Assay Principle

Under alkaline condition, colorless p-nitrophenol is converted to 4-nitrophenoxide, which develops a very intense yellow color. Its intensity is proportional to the activity of alkaline phosphatase. Alkaline phosphatase

p-nitrophenyl phosphate + H2O  p-nitrophenol + phosphate

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References
  • Haussament T.U. et. al. Clin. Chem. Acta 35, 271273 (1977)
  • Zilva JF, Pannall PR, “Plasma Enzymes in Diagnosis” in Clinical Chemistry in Diagnosis and Treatment. Lloyd London 1979:Chap 15 343.
  • IFCC method for the measurement of ALP J Clin Chem Clin Biochem1983: 21:731-48.
  • Young DS. Effects of Drugs on Clinical Laboratory Tests. Third Edition 1990: 3: 19-25.
  • Tietz Textbook of Clinical Chemistry, Second Edition, WB Saunders1994;830 – 843.
  • Wachtel M et al, Creation and Verification of Reference Intervals. Laboratory Medicine 1995; 26:593-7.
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