Aldosterone

The Aldosterone ELISA is an enzyme immunoassay for measurement of aldosterone in serum, plasma and urine.


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Product Catalog No: EIA-5298 Pack Size: 96 Wells

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Summary

The steroid hormone aldosterone is a potent mineral corticoid that is produced by the zona glomerulosa of the adrenal cortex in the adrenal gland. The synthesis and release are controlled by the renin-angiotensin-aldosterone system (RAAS)1, as well as by plasma potassium concentration 2, the pituitary peptide ACTH, and by the blood pressure via pressure sensitive baroreceptors in the vessel walls of nearly all large arteries of the body 3. Aldosterone binds to mineralocorticoid receptors (MR) and triggers the transcription of hormone-responsive genes. In consequence, aldosterone increases the blood pressure by reabsorption of sodium and water from the distal tubules of the kidney into the blood, secretion of potassium into the urine, and elevation of circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Aldosterone activity is reduced in Addison’s disease and increased in Conn’s syndrome.

Test Principle

The DRG Aldosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.

The microtiter wells are coated with a polyclonal rabbit antibody directed towards an antigenic site of the aldosterone molecule. Endogenous aldosterone of a specimen sample competes with an aldosterone-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.

After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of aldosterone in the specimen sample.

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References
  • Brown RD, Strott CA, and Liddle GW. Site of stimulation of Aldosterone biosynthesis by angiotensin and potassium. J Clin Invest. (1972), 51 (6), 1413–8.
  • Bauer JH, Gauntner WC. Effect of potassium chloride on plasma renin activity and plasma aldosterone during sodium restriction in normal man. Kidney Int. (1979), 15 (3): 286–93.
  • Williams GH, Dluhy RG. Aldosterone biosynthesis. Interrelationship of regulatory factors. Am J Med. (1972), 53 (5), 595–605.
  • Tiu SC et al. The use of aldosterone-renin ratio as a diagnostic test for primary hyperaldosteronism and its test characteristics under different conditions of blood sampling. J Clin Endocrinol Metab. (2005), 90 (1), 72-8.
  • Mulatero P et al. Confirmatory tests in the diagnosis of primary aldosteronism. Horm Metab Res. (2010), 42 (6), 406-10.
  • Quillo AR. Primary aldosteronism: results of adrenalectomy for nonsingle adenoma. J Am Coll Surg. (2011), 213 (1), 106-12.
  • Grossmann C and Gekle M. New aspects of rapid aldosterone signaling. Mol Cell Endocrinology (2009), 308 (1-2), 53-62.
  • Thomas L (editor). Renin-Angiotensin-Aldosteron-System (RAAS). Labor und Diagnose (2005); 1406-24.
  • Perschel FH et al. Rapid Screening test for primary hyperaldosteronism: ratio of plasma aldosterone to renin concentration determined by fully automated chemiluminescence immunoassays. Clin. Chemistry (2004); 50 (9), 1650-55
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