Adenovirus IgA

The DRG Adenovirus IgA Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgA-class antibodies to Adenovirus in serum.

Regulatery Status: RUO
Catalog No Size
Product Catalog No: EIA-3445 Pack Size: 96 Wells

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Test Principle

The DRG Adenovirus IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Adenovirus antigen. Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Adenovirusspecific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Adenovirus-specific IgA antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

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References
  • Donelli,G.,F.Superti,A.Tinari et al.: Viral childhood diarrhoe in Rome: a diagnostic and epidemiological study.Microbiologica 16 (1993) 215-225
  • Adrian,T.,J.Sassinek,R.Wiegand:Genome type analysis of 480 isolates of adenovirus type 1,2 and 5.Arch Virol.112 (1990) 235-248
  • Hierholzer,J.C.: Adenovirus in the immunocompromised host. Clin.Microbiol.Rev.5 (1992) 262-274
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