25-OH Vitamin D Assay for CCA
25-OH Vitamin D Assay is designed for the quantification of total 25-OH Vitamin D in serum and plasma samples.
Vitamin D Assay for Clinical Chemistry Analyzers is a three liquid stable reagent system which provides reliable results. The method requires only 20 μL of sample, completed in less than 19 minutes, and has excellent correlation with existing commercial products with R2 of 0.963, a slope of 0.912 and a y-intercept of 5.645. The assay has within-run precision values of 8.7% and a between-run precision value of 9.1%.
The test is based on the principle of a-complementation of the enzyme β-galactosidase and the competition between an enzyme donor-25-OH Vitamin D conjugate, an antiVitamin D antibody and the 25-OH Vitamin D content of a serum sample. Samples with higher 25-OH Vitamin D concentrations produce higher β-galactosidase activities and vice versa. A nitrophenyl-ß- galactoside derivative (NPG) is used as the enzyme substrate. The reaction’s product has maximum absorbance at 415 nm. The 25-OH Vitamin D concentration of a sample is proportional to the measured β-galactosidase activity.