17-α-OH Progesterone ELISA

The 17-α-OH Progesterone ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of 17-α-OH Progesterone (17-α-OHP) in serum


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Product Catalog No: EIA-1292 Pack Size: 96 Wells

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Summary

The steroid 17-α-Hydroxyprogesterone (17-α-OHP) is produced by both the adrenal cortex and gonads. Even though 17α-OHP has relatively little progestational activity, it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Because Cpd-S is produced by 21-hydroxylation of 17-α-OHP, measurement of 17-α-OHP is a useful indirect indicator of 21-hydroxylase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17-α-OHP is secreted in abundant excess. It is moderately elevated in the 11-βhydroxylase deficiency as well. Measurement of 17-α-OHP is therefore valuable in the initial diagnosis of CAH.

Test Principle

The DRG 17-α-OH Progesterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.

The microtiter wells are coated with a polyclonal [goat] antibody directed towards an antigenic site of the 17-α-OHP molecule. Endogenous 17-α-OHP of a patient sample competes with a 17-α-OHP-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of 17-α-OHP in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of 17-α-OHP in the patient sample.

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References
  1. Abraham, G.E., R.S. Swerdloff, D. Tulchinsky et al: Radioimmunoassay of plasma 17-hydroxyprogesterone. J. Clin. Endocrinol. Metab. 33:42, 1971
  2. Chrousos, G.P., D. L. Loriaux, D.L. Mann, et al: Late onset 21- hydroxylase deficiency mimicking idiopathic hirsutism or polycystic avarian disease. Annals Intern. Med. 96:143, 1982.
  3. Buster, J.E., R.J. Chang, D.L. Preston, et al: Interrelationships of circulating maternal steroids; progesterone, 16αhydroxyprogesterone, 17α-hydroxyprogesterone, 20α-dihydroprogesterone, gamma-5-pregnolonone, gamma-5- pregnolonone-sulfate, gamma-5-pregnolone-sulfate and 17-hydroxy gamma-5-pregnolonone, J. Clin. Endocrinol. Metab. 48:133, 1979.
  4. New, M.I., B. Dupont, S. Pang, et al: An update on congenital adrenal hyperplasia. Recent Progress in Hormone Research, 37:105, 1981. 5. Pang S.,
  5. J. Hotchkiss, A. Drash, et al: Micro filter paper method for 17α-hydroxyprogesterone radioimmunoassay: Its application for rapid screening for congenital adrenal hyperplasia. J. Clin. Endocrinol. Metab., 45:1003, 1977.
  6. Lobo, R.A., U. Goebelsmann: Adult manifestation of congenital adrenal hyperplasia due to incomplete 21hydroxylase deficiency mimicking polycystic ovarian disease. Am. J. Obstet. Gynecol., 138:720, 1980.
  7. Urban, M.D., P.A. Lee and C.J. Migeon: Adult high infertility in men with congenital adrenalized hyperplasia. N. Engl. J. Med. 299:1392, 1978.
  8. Meikle, A.W., R.J. Worley and C.D. West: Adrenal corticoid hyper-response in hirsute women. Fertil. Steril. 41:575, 1984
  9. Ueshiba, H., Zerah M., New M. I.: Enzyme-linked Immunosorbent assay (ELISA). Method for screening of nonclassical steroid 21-Hydroxylase deficiency. Norm. Metab. Res. 26:43, 1994
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